What selective media can be used to identify bacteria?

Eosin-Methylene Blue (EMB) agar is a selective & differential medium used to isolate Gram-negative enteric bacteria and distinguish between them.

What are the types of selective media?

Examples of selective media:

  • Eosin methylene blue contains dyes that are toxic for Gram-positive bacteria.
  • YM (yeast extract agar) has a low pH, deterring bacterial growth.
  • MEA (malt extract agar) has a low pH, deterring bacterial growth.
  • MacConkey agar is for Gram-negative bacteria.

Which media is used as a selective media?

Media that inhibit the growth of unwanted microorganisms and support the growth of the organism of interest by supplying nutrients and reducing competition are called selective media. An example of a selective medium is MacConkey agar.

What are the types of bacterial culture media?

These are classified into six types: (1) Basal media, (2) Enriched media, (3) Selective (4) Indicator media, (5) Transport media, and (6) Storage media. 1. BASAL MEDIA. Basal media are those that may be used for growth (culture) of bacteria that do not need enrichment of the media.

Is nutrient agar a selective media?

Notice that nutrient agar is neither a selective nor differential medium.

What is the media for bacteria?

Examples of standard general purpose media that will support the growth of a wide variety of bacteria include nutrient agar, tryptic soy agar, and brain heart infusion agar. A medium may be enriched, by the addition of blood or serum.

What are selective media?

Selective media are used to select for the growth of a particular “selected” microorganism. For example, if a certain microbe is resistant to aparticular antibiotic (e.g., novobiocin), then that antibiotic can be added to the medium in order to prevent other organisms, which are not resistant, from growing.

Is EMB agar selective or differential?

EMB agar contains peptone, lactose, sucrose, and the dyes eosin Y and methylene blue; it is commonly used as both a selective and a differential medium. EMB agar is selective for gram-negative bacteria.

Is blood agar selective?

Blood agar is a non-selective medium that can be made selective for certain pathogens by the addition of antibiotics, chemicals or dyes.

How do you identify different bacteria?

Bacteria are identified routinely by morphological and biochemical tests, supplemented as needed by specialized tests such as serotyping and antibiotic inhibition patterns. Newer molecular techniques permit species to be identified by their genetic sequences, sometimes directly from the clinical specimen.

Is EMB a selective media?

What is selective media for E. coli?

An enriched lauryl sulphate-aniline blue agar medium which is selective for Escherichia coli and coliform organisms is described. From faecal samples, the medium gave higher counts of colonies producing acid from lactose than media containing bile salts.

What are some examples of selective media?

Selective media allows for the growth of one type of bacteria while preventing the growth of another type. An example of selective media that was used in this experiment is the Eosin Methylene-Blue agar used for the growth of Escherichia coli. These bacteria are commonly found in places of fecal contamination (CDC).

What are the different types of media in microbiology?

Eosin methylene blue (EMB) that contains methylene blue – toxic to Gram-positive bacteria,allowing only the growth of Gram negative bacteria.

  • YM (yeast and mold) which has a low pH,deterring bacterial growth.
  • MacConkey agar for Gram-negative bacteria.
  • Hektoen enteric agar (HE) which is selective for Gram-negative bacteria.
  • What is the difference between selective and differential media?

    The main difference between selective and differential media is that selective media are used to isolate a particular strain of microorganisms whereas differential media are used to identify and differentiate a closely-related group of microorganisms.

    What is selective medium in microbiology?

    What is a selective medium in microbiology? Selective Media. The next type of media is selective media. A selective media is composed of specific ingredients to inhibit the growth of certain species of microbes in a mixed culture while allowing others to grow. Again, in order to fully grasp this definition, let’s look at an example of a