What is Nichols strain Treponema pallidum?

It is an obligate human parasite. Strain Nichols was isolated in 1912 from the cerebrospinal fluid of a patient with secondary syphilis. It has been passed in rabbits since 1912.

What is Nichols strain?

The Nichols strain of virulent T. pallidum, isolated and maintained by serial transmission in live rabbit testes, is being used as the source of specific antigen in the Treponemal Immobilization (TPI) and Treponemal Agglutination (TPA) tests for syphilis.

What is Reiter Treponemes?

Abstract. A sonicate of Treponema phagedenis biotype Reiter (Reiter treponemes) was used as sorbing agent in parallel with sorbent in the FTA-ABS test. By diluting the Reiter treponemal antigens in approximately 0.5 M NaCl, the Reiter sonicate could be produced in sufficient quantities for its use in routine FTA-ABS.

What is Reiter’s strain?

no doubt that the so-called “Reiter strain” is a. genuine Treponema pallidum. Altogether, nineto. eleven strains were isolated during that time in the manner described below. By these experiments some strains were found to behave differently when inoculated into rabbit testes.

How does RPR test work?

RPR (rapid plasma reagin) is a screening test for syphilis. It measures substances (proteins) called antibodies that are present in the blood of people who may have the disease. Blood is drawn from a vein (venipuncture), usually from the inside of the elbow or the back of the hand.

Is Treponema pallidum normal flora?

Human pathogens cause four treponematoses: syphilis (T pallidum subsp pallidum), yaws (T pallidum subsp pertenue), endemic syphilis (T pallidum subsp endemicum), and pinta (T carateum). Nonpathogenic treponemes may be part of the normal flora of the intestinal tract, the oral cavity, or the genital tract.

What is RPR in syphilis?

What are the characteristics of Treponema pallidum?

Treponema pallidum is a Gram-negative micro-aerophilic bacterium, 6–20 μm long, 0.1–0.2 μm wide and tightly coiled. The treponemes are motile by three flagella (axial filaments) that wrap around the surface of the organism and are covered by the outer membrane which contains lipopolysaccharide.

What is the difference between Vdrl and syphilis?

The venereal disease research laboratory (VDRL) test is designed to assess whether you have syphilis, a sexually transmitted infection (STI). Syphilis is caused by the bacterium Treponema pallidum. The bacterium infects by penetrating into the lining of the mouth or genital area.

Why is RPR a non-treponemal test?

Non-treponemal tests, also called screening tests (RPR and VDRL), do not detect antibodies specific for syphilis and are based upon the reactivity of serum from infected patients to a cardiolipin-cholesterol- lecithin antigen (regain).

What is Treponema pallidum AB RPR Qual?

A rapid plasma reagin (RPR) test is a blood test used to screen you for syphilis. It works by detecting the nonspecific antibodies that your body produces while fighting the infection. Syphilis is a sexually transmitted infection (STI) caused by the spirochete bacterium Treponema pallidum.

What is the difference between treponemal and non-treponemal test?

Treponemal tests detect antibody to T pallidum proteins. Nontreponemal tests detect antibodies directed against lipoidal antigens, damaged host cells, and possibly from treponemes. Both tests are used to confirm the infection and determine whether the disease is active.

What is the difference of treponemal test for syphilis to non-treponemal test for syphilis?

What is the difference between RPR and VDRL?

The rapid plasma reagin (RPR) test uses the same antigen as VDRL, but the antigen is bound to a carbon particle to allow visualization of the reaction without a microscope. VDRL or RPR titers are monitored to document response to therapy. RPR titers are frequently higher than VDRL titers.

Can Treponema pallidum be cultivated under strict anaerobic condition in Smith Noguchi medium?

Certain other non-pathogenic strains (e.g. Reiter strain) can be cultivated under strict anaerobic condition in Smith Noguchi medium. 4. Viability of Treponema Pallidum (Tr.

What is the characteristic motility of Treponema pallidum?

Tr. pallidum shows rotary corkscrew like highly characteristic motility (Angulation) which can be easily seen under dark field microscope. 2. Staining of Treponema Pallidum (Tr.

Is penicillin lethal to Treponema pallidum?

Penicillin is lethal to Treponema pallidum in a concentration of 0.003 unit/ml and is the drug of choice. Related Articles: 3 Tests Employed for Serodiagnosis of Syphilis | Immunity | Microbiology

What is the causative agent of syphilis Treponema pallidum?

The causal agent of Syphilis Treponema pallidum was first discovered in 1905 by a German scientist Schaudinn in the primary sores (Chancres, pronounced shankers) of a syphilitic patient.