What is PDGF used for?
Medicine. Recombinant PDGF is used to help heal chronic ulcers and in orthopedic surgery and periodontics to stimulate bone regeneration and repair.
What is PDGF in blood?
A family of molecules released from platelets (tiny pieces of cells that are found in the blood and that help the blood clot). Forms of PDGF help to heal wounds and to repair damage to blood vessel walls. They also help blood vessels grow. Also called platelet-derived growth factor.
What does PDGF do in the cell cycle?
Platelet-derived growth factor (PDGF) is a potent mitogen for mesenchymal cells. PDGF AA functions as a “competent factor” that stimulates cell cycle entry but requires additional (progression) factors in serum to transit the cell cycle beyond the G1/S checkpoint.
Where is PDGF found?
platelet granules
PDGF is stored in platelet granules and, as such, it can act as a systemic regulator of cell function. In humans, the circulating forms of PDGF are, for the most part, PDGF BB and AB (98). Osteoblasts and osteosarcoma cells express the PDGF A and B genes (99–101).
In what form are PDGF proteins active?
PDGFs are disulfide-bonded heterodimeric proteins that act mainly on stromal cells and regulate the wound healing process. Originally isolated from platelets, PDGF isoforms are also secreted from macrophages, endothelial cells, and fibroblasts.
What is the anti-PDGF B antibody?
Overview Product name Anti-PDGF B antibody Description Rabbit polyclonal to PDGF B Host species Rabbit Specificity This antibody has been tested against PDGF BB protein (ab9706) and has yet to be tested against endogenous protein. Tested applications Suitable for: IHC-Fr, WB, IHC-Pmore details Species reactivity Reacts with: Mouse, Human
What is the function of IL-4 antibody?
IL-4 Antibodies. Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13.
What is the molecular weight of Recombinant PDGF BB?
ab23914 detects a band of ~ 14 kDa corresponding to the reduced form of recombinant PDGF BB ( ab9706 ). The predicted molecular weight for PDGF BB is 27 kDa according to the Swissprot entry, however the reduced form is known to run at between 14-18 kDa.
What is the secondary antibody to the anti-rabbit IgG antibody?
An Alexa Fluor® 647-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. DAPI (blue) – nuclei.